Services

Beyond novel epitope discovery, Pure MHC can intersect your existing drug development pipeline at a number of points:

Qualifying target candidates – Pure MHC can compare a partner’s putative targets against its proprietary database of normal tissues to determine potential for cross-reactivity.

Existing Target Validation and Tissue Distribution – Partners that have already selected antigens for immune targeting can enlist Pure MHC to validate that a target is detected in the appropriate tissues and to determine a quantitative measurement of the target’s immunogenicity.

Full Length HLA – In addition to soluble HLA, Pure MHC also has developed Full Length constructs and transfectants for Class I, Class II, and non-classical HLA applications.

Affinity Testing
Pure MHC has access to the largest array of HLA molecule competitive binding assays with 42 different HLA variants currently available (and more being developed and optimized) for testing. These assays can be used to assess the affinity of a lead candidate or for selection of candidates for HLA presentation, in order to advance a target to further development.
Antigen Tissue Distribution Studies
Using a previously identified target peptide, Pure MHC can refold that peptide into an HLA complex and generate a TCRm to that HLA-peptide complex. This TCRm can then be used to validate that target by surveying the tissue distribution of the target in a binding study with that TCRm.
Agnostic Epitope Confirmation
Further validation is afforded by conducting an agnostic survey of the HLA-peptide population from a nominated representative affected cell line and/or primary tissue of interest and verifying a) that the target peptide is presented by HLA in that cell line and/or tissue, and b) the relative abundance of that peptide among the total peptide pool.

Companion Diagnostics – Cell surface biomarkers and mAb to these biomarkers can be utilized to develop companion diagnostics to assess the effectiveness of antigen/epitope delivery and antigen/epitope presentation.

Regulatory Compliance Support – Companies with a validated target in a formulated vaccine have enlisted Pure MHC to provide companion diagnostics to demonstrate target expression on vaccinated cells and lot-to-lot QC testing for regulatory compliance purposes.

Other Custom Services – In addition to soluble HLA, Pure MHC also has developed Full Length constructs and transfectants for Class I, Class II, and non-classical HLA applications.

Learn how Pure MHC services can help you reach your scientific goals.