Epitope Discovery


HLA proteins act as an alarm system for detection of cancerous and infected cells by presenting a foreign or aberrant peptide to T cells. Using its proprietary soluble HLA-expressing cell lines, Pure MHC is able to simulate the disease state and identify the HLA peptide complexes that distinguish cancerous or infected cells.

Pure MHC’s epitope discovery technology is the only technology that can identify peptide epitopes that are both: 1) unique to or upregulated in the affected state, and 2) compared to and validated as ligands presented by HLA molecules on primary tissues of interest.

The Pure MHC Epitope Discovery Process:


  • identifies high quality targets
  • utilizes the capabilities to study all 120 Class I HLA alleles, as well as Class II, non-classical HLA molecules
  • allows access to intracellular targets
  • validates, rather than predicts, that target is “actually” expressed
  • is agnostic to previously reported targets
  • overcomes heterogeneity in tumors and pathogen strains

Epitope Discovery Competitor Analysis

Characteristics PureMHC Competitor
Class I allelesNon-classical Class I alleles

Class II alleles

Yes (121 currently available)Yes (HLA-E, HLA-F, HLA-G, CD1c, CD1d)

Yes (37 currently available)



Maximum Ligands Identified
(per allele)
>30000* <1000
MHC isolation Soluble HLA Detergent Cell Lysate
MHC restriction Known, allele specific Predicted by typing, mix of multiple alleles
Source Any transfectable cell line or primary tissue HumanMouse
Host Cell Species Any species available HumanMouse
Species MHC Human
Non-human Primates (Mamu, Mafa, Gogo, Patr)
Any other species with MHC (Fla, Cfa, Gga, etc.)
Antigen Delivery ExogenousEndogenous (Pathogen Infection) Exogenous
Peptide Origin Purified ProteinVaccine

Pathogens (up to BSL III)

Small molecule drugs

Endogenous Self Peptides

Purified ProteinVaccine
Comparative analysis Yes No
Motif analysis Yes No
Disease Applications AllergyBiologics

Infectious Disease



Drug Hypersensitivity

Binding Motifs


Autoimmunity (probably)

* Typical results for soluble Class I HLA from cell lines; may depend on allele. Note: Publications available for reference.


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